Description
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-35 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-35 and IL-35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve